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Updated: Jul 13, 2026
10:22
Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Published on: November 12, 2015
The role of pre-replicative complex (pre-RC) components in oncogenesis
Eric Lau1, Toshiya Tsuji, Liping Guo
1The Burnham Institute for Medical Research, 10901 North Torrey Pines Road, La Jolla, CA 92037, USA.
Summary
Prereplicative complex (pre-RC) proteins regulate DNA replication. Deregulation of these proteins can lead to genomic instability and tumorigenesis, suggesting their potential as cancer biomarkers and therapeutic targets.
Area of Science:
- Molecular Biology
- Cell Cycle Regulation
- Oncology
Background:
- Normal DNA replication is tightly controlled to occur once per cell cycle.
- Dysregulation of DNA replication leads to genomic instability, implicated in cancer and developmental defects.
- Prereplicative complex (pre-RC) proteins are key regulators of DNA replication initiation.
Purpose of the Study:
- To review evidence on the role of pre-RC proteins in oncogenesis.
- To explore the potential of pre-RC proteins as diagnostic and therapeutic targets in cancer.
Main Methods:
- Literature review of studies on pre-RC proteins and cancer.
- Analysis of data from organismal models with pre-RC protein deregulation.
- Examination of the correlation between pre-RC protein levels, tumor grade, and prognosis.
Main Results:
- Overexpression of several pre-RC proteins is observed in cancer, serving as potential tumor markers.
- Deregulation of individual pre-RC proteins (overexpression or deficiency) consistently perturbs cell cycle regulation and causes genomic instability.
- Evidence suggests pre-RC proteins may play active roles in tumorigenesis, beyond just indicating proliferation.
Conclusions:
- Pre-RC proteins are implicated in oncogenesis through their roles in cell cycle control and genomic stability.
- Pre-RC proteins may possess significant diagnostic and therapeutic value in cancer management.
- Further research is warranted to fully elucidate the oncogenic potential and clinical utility of pre-RC proteins.
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The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
02:38S-Cdk Initiates DNA Replication
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of replication.
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