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Isolation and Culture Expansion of Tumor-specific Endothelial Cells
Published on: October 14, 2015
Dendritic cell-endothelial cell cross-talk in angiogenesis
Silvano Sozzani1, Marco Rusnati, Elena Riboldi
1Unit of General Pathology and Immunology, Department of Biomedical Sciences and Biotechnology, University of Brescia, Viale Europa 11, 25123 Brescia, Italy. sozzani@med.unibs.it
Trends in Immunology
|August 19, 2007
Summary
Dendritic cells (DCs) influence adaptive immunity and inflammation. Certain DC subsets promote blood vessel growth (angiogenesis) via vascular endothelial growth factor-A (VEGF-A), impacting various physiological conditions.
Area of Science:
- Immunology
- Cell Biology
- Angiogenesis Research
Background:
- Dendritic cells (DCs) are key antigen-presenting cells regulating adaptive immunity and inflammation.
- DCs share pathogen-killing and cytokine-release functions with other phagocytes.
- DCs can modulate inflammation through cytokine and chemokine release.
Purpose of the Study:
- To investigate the role of dendritic cell subsets in producing pro- and anti-angiogenic mediators.
- To explore the influence of vascular endothelial growth factor-A (VEGF-A) in DC-mediated angiogenesis.
- To understand the bidirectional relationship between angiogenic mediators and DC biology.
Main Methods:
- Analysis of cytokine and chemokine profiles from different dendritic cell subsets.
- Assessment of dendritic cell activation status and its effect on mediator release.
- Investigation of the impact of pro- and anti-angiogenic factors on dendritic cell differentiation and maturation.
Main Results:
- Specific dendritic cell subsets release diverse pro- and anti-angiogenic factors.
- Alternatively activated DCs exhibit significant pro-angiogenic activity, primarily via VEGF-A.
- Angiogenic mediators influence dendritic cell differentiation and maturation processes.
- Dendritic cells possess the capacity for trans-differentiation into endothelial-like cells.
Conclusions:
- Dendritic cells play a significant role in regulating angiogenesis through the production of specific mediators like VEGF-A.
- The interaction between dendritic cells and angiogenic factors is bidirectional, affecting DC biology.
- Dendritic cells may contribute to neovascularization in various physiopathological contexts, including potential roles in vasculogenesis.
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