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Protein release from biodegradable dextran nanogels.

T G Van Thienen1, K Raemdonck, J Demeester

  • 1Laboratory of General Biochemistry and Physical Pharmacy, Department of Pharmaceutics, Ghent University, Harelbekestraat 72, 9000 Ghent, Belgium.

Langmuir : the ACS Journal of Surfaces and Colloids
|August 19, 2007
PubMed
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Biodegradable dextran nanogels, created using liposomes, effectively encapsulate and control the release of proteins like bovine serum albumin (BSA) and lysozyme for intracellular delivery. These nanogels show promise for drug delivery due to their stability in serum and potential for lyophilization.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery Systems

Background:

  • Intracellular drug delivery requires efficient systems for targeting specific cellular sites.
  • Biodegradable nanogels offer potential for controlled release of therapeutic agents.

Purpose of the Study:

  • To develop and characterize biodegradable dextran nanogels for controlled intracellular protein release.
  • To evaluate the encapsulation efficiency, release kinetics, and stability of these nanogels.

Main Methods:

  • Preparation of dextran nanogels via UV polymerization of dextran hydroxyethylmethacrylate (dex-HEMA) using liposomes as nanoreactors.
  • Encapsulation of proteins like bovine serum albumin (BSA) and lysozyme.
  • Analysis of protein release rates influenced by cross-link density and lipid coating.

Related Experiment Videos

  • Assessment of nanogel aggregation in human serum and stability after lyophilization.

    • Main Results:

    • Dextran nanogels achieved approximately 50% encapsulation efficiency for BSA and lysozyme.
    • Protein release kinetics were modulated by nanogel cross-link density and lipid coating, occurring over days to weeks.
    • Nanogels demonstrated stability in human serum and successful reconstitution from lyophilized form.
    • Cellular uptake indicates potential for intracellular applications.

    Conclusions:

    • Biodegradable dextran nanogels are effective carriers for controlled intracellular protein delivery.
    • The nanogel properties, including cross-link density and lipid coating, allow tunable protein release.
    • These nanogels exhibit favorable characteristics for intravenous administration and storage, highlighting their therapeutic potential.