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Related Experiment Video

Updated: Jul 13, 2026

Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper

Preparation of Expanded Chitin Foams and their Use in the Removal of Aqueous Copper

Published on: February 27, 2021

Chitosan adsorption to salivary pellicles.

Henny C van der Mei¹, Eefje Engels, Joop de Vries

¹Department of Biomedical Engineering, University Medical Center Groningen and University of Groningen, 9700 AD Groningen, The Netherlands. h.c.van.der.mei@med.umcg.nl

European Journal of Oral Sciences

|August 19, 2007

Summary

Chitosans adsorb to salivary pellicles, altering surface charge and hydrophobicity. This modification of the protein film suggests chitosans can anchor antimicrobials to oral surfaces.

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Formation of Human Periodontal Ligament Cell Spheroids on Chitosan Films

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Published on: June 19, 2019

Area of Science:

Biomaterials Science
Surface Chemistry
Oral Biology

Background:

The salivary pellicle, a protein film on teeth, readily attracts oral bacteria.
Chitosans are cationic biomolecules with antimicrobial properties that can be modified.

Purpose of the Study:

To investigate how chitosans interact with and alter the properties of salivary pellicles.
To assess the potential of chitosans as anchoring agents for antimicrobials.

Main Methods:

X-ray photoelectron spectroscopy (XPS) for surface chemical composition.
Water contact angle measurements for hydrophobicity.
Streaming potential measurements for surface charge.
Atomic force microscopy (AFM) for surface morphology.

Main Results:

Chitosan adsorption was confirmed by XPS, showing altered carbon functionalities and a characteristic N(1s) peak.
Chitosan treatment resulted in a more hydrophobic and positively charged pellicle surface.
AFM revealed increased pellicle surface roughness due to protein aggregation after chitosan treatment.

Conclusions:

Chitosans significantly alter salivary pellicle surface properties, including charge, hydrophobicity, and morphology.
The adsorption of chitosans to pellicles indicates their potential as effective anchoring molecules for antimicrobials.