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Related Experiment Video

Updated: Jul 13, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
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Xylitol conversion by fermentation using five yeast strains and polyelectrolyte-assisted ultrafiltration.

Chun-Han Ko1, Pei-Chun Chiu, Chin-Lin Yang

  • 1School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan. chunhank@ntu.edu.tw

Biotechnology Letters
|August 19, 2007
PubMed
Summary

Candida tropicalis demonstrated the highest xylitol (a sugar alcohol) production from lignocellulosic hydrolysates. This yeast achieved a 0.79 g/g bioconversion yield, showing potential for efficient xylitol biosynthesis.

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Genetic Engineering of an Unconventional Yeast for Renewable Biofuel and Biochemical Production

Published on: September 20, 2016

Area of Science:

  • Biotechnology
  • Microbiology
  • Biochemical Engineering

Background:

  • Xylitol is a valuable sugar alcohol with diverse applications.
  • Lignocellulosic biomass is a sustainable feedstock for producing chemicals like xylitol.
  • Efficient microbial strains and fermentation processes are crucial for cost-effective xylitol production.

Purpose of the Study:

  • To evaluate xylitol production from lignocellulosic hydrolysates using various yeast strains.
  • To optimize fermentation conditions for maximizing xylitol yield.
  • To investigate methods for recovering xylitol from fermentation broth.

Main Methods:

  • Batch fermentations were performed using five yeast strains: Candida boidinii, C. guilliermondii, C. tropicalis, C. utilis, and P. anomala.
  • A sugar mixture simulating lignocellulosic hydrolysates served as the carbon source.
  • Cross-flow ultrafiltration with polydiallyldimethylammonium chloride treatment was used for xylitol recovery.

Main Results:

  • Candida tropicalis exhibited the highest bioconversion yield (Y(P/S)) of 0.79 g/g over 48 hours.
  • Optimized fermentation with C. tropicalis yielded 0.6 g/g and 0.39 g/g using urea and soybean meal as nitrogen sources, respectively.
  • Ethanol and arabitol were co-produced. Ultrafiltration reduced protein contamination in the permeate by 79%.

Conclusions:

  • Candida tropicalis is a promising yeast strain for efficient xylitol production from lignocellulosic hydrolysates.
  • Fermentation conditions, including nitrogen source, significantly impact xylitol yield.
  • Ultrafiltration is an effective method for purifying xylitol from fermentation broth.