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Isolation and molecular characterization of a cryptic plasmid from Bifidobacterium longum
Young-Seo Park1, Ki-Hwan Kim, Ju Hui Park
1Department of Food Science and Biotechnology, Kyungwon University, Seongnam 461-701, Korea.
Abstract:
An isolate from the fecal samples of children was identified as Bifidobacterium longum. A plasmid isolated from it pBIFA24 was 4,892 bp with three open reading frames, ORFI, ORFII, and ORFIII. ORFI encoded a replication protein involved in a rolling-circle replication mechanism, and three sets of tandem repeat sequences featuring iteron structure were identified. Secondary structure prediction analysis of ORFII suggested it was a transmembrane protein. ORFIII showed high amino acid sequence identity with some mobilization proteins and contained an oriT sequence.
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