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Black substrate for spectrophotometric determination of cellulase activity in coloured solutions
1Laboratory of Analytical Chemistry, South Bohemian Biological Centre, Ceské Budĕjovice, Czechoslavakia.
Journal of Biochemical and Biophysical Methods
|December 1, 1991
Abstract:
A simple procedure for spectrophotometric determination of cellulase activity in coloured solutions is described. CM-cellulose, cross-linked with epichlorhydrin in the presence of black drawing ink, is used as an insoluble chromolytic substrate. The absorbance of reaction mixture filtrates are read in the near infra-red region (at 800-900 nm) where the absorbances of contaminating coloured substances are substantially lowered; by contrast, black drawing ink released from the substrate during the action of cellulases absorbs strongly at these wavelengths.