Kinetic analysis of human IL-2 activated cytotoxic cells

Kinetic analysis was used to define lytic events in peripheral blood mononuclear cells (PBMC) activated in lymphokine conditioned medium (LCM) and recombinant interleukin-2 (rIL-2). This analysis provided quantitative information on the functional properties of these lymphokine-activated killer (LAK) cells against NK-resistant and NK-sensitive tumor cell lines. The maximum rate of target cell lysis (Vmax) and Km (target cell number resulting in 1/2 Vmax) were determined. IL-2 activated effector cells that bound to target cells also lysed them (i.e., non-lytic bystander lymphocytes did not influence the determination of kinetic parameters) in contrast to lysis mediated by unactivated NK cells. The extent of LAK cell binding to tumor target cells was dependent upon the tumor type. LAK cell frequency determinations were calculated where Km approximated the concentration of LAK cells that were capable of killing a particular target. LAK cells generated in rIL-2 were lytically more efficient than those activated in LCM, and T-depletion resulted in a LAK population with the highest maximum rate of lysis. The use of kinetic analysis to evaluate LAK cell frequencies and quantitate lytic events will be useful in determining the effects of drugs, biological response modifiers and disease states on LAK cell function.