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Updated: Jul 13, 2026

Derivation of Human Embryonic Stem Cells by Immunosurgery
Published on: December 13, 2007
Derivation of human embryonic stem cells from single blastomeres
Irina Klimanskaya1, Young Chung, Sandy Becker
1Advanced Cell Technology, Worcester, Massachusetts 01605, USA.
Abstract:
This protocol details a method to derive human embryonic stem (hES) cells from single blastomeres. Blastomeres are removed from morula (eight-cell)-stage embryos and cultured until they form multicell aggregates. These blastomere-derived cell aggregates are plated into microdrops seeded with mitotically inactivated feeder cells, and then connected with neighboring microdrops seeded with green fluorescent protein-positive hES cells. The resulting blastomere-derived outgrowths are cultured in the same manner as blastocyst-derived hES cells. The whole process takes about 3-4 months.
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