Related Experiment Video
Updated: Jul 13, 2026
Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
Published on: August 10, 2022
Human parthenogenetic blastocysts derived from noninseminated cryopreserved human oocytes
Ester Polak de Fried1, Pablo Ross, Gisela Zang
1Department of Reproductive Medicine, CER Medical Institute, School of Medicine, Buenos Aires University, Buenos Aires, Argentina. ester_polak@cermed.com
Objective:
To report on the development of human parthenogenetic blastocysts and an in vitro attachment that was generated from noninseminated cryopreserved human oocytes for the first time.
Design:
Prospective study.
Setting:
Department of reproductive medicine in a medical institute in Buenos Aires, Argentina.
Patient(S):
Five healthy fertile donors.
Intervention(S):
Artificial activation of noninseminated cryopreserved human oocytes after thawing, parthenote culture, and their in vitro attachment.
Main Outcome Measure(S):
Survival rate, activation rate, cleavage rate, and blastocyst formation.
Result(S):
Thirty-six of 38 cryopreserved noninseminated oocytes survived after thawing (survival rate, 94.7%). Thirty-one of 36 oocytes showed one pronucleus (activation rate, 86.1%). Thirty of 31 cleaved (cleavage rate, 96.8%). Five of 30 showed cavitation (blastocyst rate, 16.7%).
Conclusion(S):
Noninseminated cryopreserved human oocytes showed a high survival rate after thawing. They responded very satisfactorily to artificial activation, which was followed by a high rate of parthenogenetic embryos, which can develop into blastocysts. In the future, these could be a new source for development of human parthenogenetic stem cells.
