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Published on: September 6, 2024
Ultrashort electric pulse induced changes in cellular dielectric properties
Allen L Garner1, George Chen2, Nianyong Chen3
1Bioelectromagnetism Laboratory, Department of Nuclear Engineering and Radiological Sciences, University of Michigan, Ann Arbor, MI 48109, USA; Frank Reidy Research Center for Bioelectrics, Old Dominion University, Norfolk, VA 23510, USA.
Nanosecond pulsed electric fields (nsPEFs) alter cell electrical properties. Jurkat cell conductivity decreased post-pulsing, indicating electropermeabilization and potential long-term membrane damage.
Area of Science:
- Biophysics
- Cell Biology
- Electroporation
Background:
- Understanding cell electrical properties is crucial for modeling nanosecond pulsed electric field (nsPEF) interactions.
- Jurkat cells, a human T-cell line, are a relevant model for studying nsPEF effects.
Purpose of the Study:
- To measure the dielectric properties of Jurkat cells before and after nsPEF exposure.
- To investigate the impact of nsPEFs on intracellular and membrane conductivity.
Main Methods:
- Time domain dielectric spectroscopy was employed to assess dielectric properties.
- Jurkat cells were exposed to five 10ns, 150kV/cm electrical pulses.
Main Results:
- Cytoplasm and nucleoplasm conductivities significantly decreased after nsPEF exposure.
- Increased cell suspension conductivity suggests ion transport out of the cell (electropermeabilization).
- A delayed decrease in cell membrane conductivity was observed, hinting at ion channel damage or use-dependent effects.
Conclusions:
- nsPEFs induce electropermeabilization in Jurkat cells, altering their electrical properties.
- The observed changes suggest both immediate ion transport and potential long-term effects on cell membrane integrity.
- This data provides valuable insights for refining models of nsPEF-cell interactions.
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