Related Experiment Video

Updated: Jul 12, 2026

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils
09:44

ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

Published on: January 3, 2025

STAT5 is an ambivalent regulator of neutrophil homeostasis

Laurence Fiévez1, Christophe Desmet, Emmanuelle Henry

  • 1Laboratory of Cellular and Molecular Physiology, GIGA-Research, University of Liège, Liège, Belgium.

Plos One
|August 22, 2007
PubMed
Abstract

Insights

Signal transducer and activator of transcription 5 (STAT5) plays a dual role in neutrophil regulation. STAT5 deficiency impairs neutrophil survival during inflammation, while its absence in liver endothelial cells promotes granulopoiesis.

Area of Science:

  • Hematology
  • Immunology
  • Molecular Biology

Background:

  • STAT5 is known to promote hematopoietic progenitor survival.
  • STAT5-deficient mice exhibit mild neutrophilia, suggesting a complex role in hematopoiesis.

Purpose of the Study:

  • To elucidate the role of STAT5 in neutrophil homeostasis and granulopoiesis.
  • To investigate the function of STAT5 in liver endothelial cells (LECs) and granulocytic lineage cells.

Main Methods:

  • Analysis of STAT5-deficient (STAT5-/-) mice.
  • Assessment of G-CSF secretion by LECs.
  • Evaluation of neutrophil production and survival under inflammatory conditions.

Main Results:

  • STAT5-/- mice show autonomous G-CSF secretion by LECs, compensating for myeloid progenitor defects.
  • Mutant mice exhibit impaired neutrophil production and survival upon inflammatory stimulation.
  • Wild-type mice demonstrate STAT5 degradation in LECs, leading to G-CSF production and neutrophil mobilization during inflammation.

Conclusions:

  • STAT5 has an ambivalent role: promoting granulocytic survival but inhibiting G-CSF expression in LECs.
  • STAT5 acts as a negative regulator of granulopoiesis in LECs by repressing G-CSF.
  • STAT5 is crucial for maintaining neutrophil homeostasis, particularly during inflammatory responses.

More Related Videos

Rapid Magnetic-microbead Method for Efficient Purification of Low-density Neutrophils08:14

Rapid Magnetic-microbead Method for Efficient Purification of Low-density Neutrophils

Published on: November 11, 2025

Related Concept Videos

The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
Inflammatory Response01:28

Inflammatory Response

An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Regulation of Bacterial Virulence01:28

Regulation of Bacterial Virulence

Pathogenic bacteria employ a range of regulatory mechanisms to modulate the expression of virulence genes in response to environmental and host-derived signals. These mechanisms ensure that virulence factors are expressed only under favorable conditions, thereby optimizing infection and survival strategies.Mechanisms of Virulence RegulationKey regulatory strategies include:Two-Component Systems: These consist of a membrane-bound sensor kinase and a cytoplasmic response regulator. Environmental...
Other Stress Responses in Bacteria01:30

Other Stress Responses in Bacteria

Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...