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Related Experiment Video

Updated: May 22, 2026

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

AdamiForge: a modular reverse genetics tool for human adenovirus type 12 (HAdV-A12).

Maxence Collard1, Jean-François Antoine1, Nicolas Debortoli2,3

  • 1Integrated Veterinary Research Unit (URVI), University of Namur, Namur Research Institute for Life Sciences (NARILIS), Namur, Belgium.

Microbiology Spectrum
|May 21, 2026
PubMed
Summary

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Researchers developed AdamiForge, a new reverse genetics tool for engineering human adenovirus A12 (HAdV-A12). This tool facilitates the study of HAdV-A12, expanding research beyond the commonly studied HAdV-C5 strain.

Area of Science:

  • Virology
  • Molecular Biology
  • Genetic Engineering

Background:

  • Human adenoviruses (HAdVs) encompass over 100 genotypes causing diverse infections.
  • Current research predominantly focuses on HAdV-C5, with limited tools for other genotypes like HAdV-A12.
  • Strain-specific characteristics impact HAdV pathogenicity and vector applications.

Purpose of the Study:

  • To develop a novel reverse genetics system, AdamiForge, for engineering the less-characterized HAdV-A12.
  • To create tools enabling the study of HAdV-A12, which is phylogenetically distant from HAdV-C5.
  • To facilitate research on HAdV genotypes beyond HAdV-C5 for broader understanding and application.

Main Methods:

  • AdamiForge divides the HAdV-A12 genome into fragments cloned into plasmids.
Keywords:
AdamiForgeHAdV-A12Mastadenovirus adamiadenovirus A12recombinant adenovirusreverse genetics

More Related Videos

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
13:17

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Published on: January 28, 2016

Related Experiment Videos

Last Updated: May 22, 2026

An Efficient Method for Adenovirus Production
10:06

An Efficient Method for Adenovirus Production

Published on: June 10, 2021

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5
13:17

Cloning and Large-Scale Production of High-Capacity Adenoviral Vectors Based on the Human Adenovirus Type 5

Published on: January 28, 2016

  • Genomic fragments are released and reassembled *in vitro* using Gibson assembly.
  • Reconstituted genomes are introduced into permissive cells to generate new virions.

    • Main Results:

    • Successfully reconstituted wild-type HAdV-A12 using the AdamiForge system.
    • Engineered recombinant HAdV-A12 expressing modified DNA-binding proteins (DBPs) fused to eGFP or FLAG-tag.
    • Demonstrated successful detection of HAdV-A12 DBP via Western blotting and immunofluorescence using engineered tags.

    Conclusions:

    • AdamiForge provides a valuable reverse genetics tool for engineering HAdV-A12.
    • The developed tools, including tagged DBPs, overcome limitations in antibody availability for HAdV-A12.
    • This work expands the toolkit for studying diverse HAdV genotypes, crucial for understanding pathogenesis and vector development.