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Published on: May 5, 2020
[Effect of nerve growth factor on mitochondrion dysfunction of motor neuron induced by 2,5-hexanedione]
Yanshu Zhang1, Qingjun Liu, Qiang Liu
1Institute of Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, China.
Objective:
To explore the effect of nerve growth factor (NGF) on mitochondrion dysfunction of motor neuron induced by 2,5-hexanedione (2,5-HD).
Methods:
VSC4.1 cells (a cell line from motor neuron) were incubated 2,5-HD at the doses of 10mmol/L and 20mmol/L. VSC4.1 were incubated with 20mmol/L 2,5-HD for 12 hours and then 2,5-HD and serum were removed and same cells were cultured with NGF-contained medium. The cell viability was measured by MTF method. The mitochondrion membrane potential (Delta psi m) was detected by Rho-123 and expression of NGF protein was detected by immunofluorescence and flow cytometry.
Results:
VSC4.1 cells were treated with 2,5-HD at doses of 10mmol/L and 20mmol/L for 12 hours, when compared with the control group, the VSC4.1 cell viabilities decreased 17% and 23% , and expressions of NGF of VSC4.1 cells also decreased (33.5% , 34%), and levels of mitochondrion membrane potential (Delta psi m) of VSC4. 1 cells decreased from 39.36 to 29.57 and 23.80, respectively. 50 and 100ng/ml NGF were added into the culture medium when VSC4.1 cells were treated with 2,5-HD at the doses of 20mmol/L for 12h. The viabilities increased 6.38% and 23.40%. Mitochondria membrane potential of VSC4.1 cells increased(from 26.03 to 30.18 and 31.50).
Conclusion:
The improvement of the mitochondrion dysfunction induced by 2,5-HD could be associated the mitochondrion membrane potential (Delta psi m) increased by NGF.
