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[Determination of total N-nitroso compounds in sausage on the market]
Kun Lin1, Shijiang Yu, Yongning Wu
1Shantou University Medical College, Shantou 515041, China. klin@stu.edu.cn
Objective:
To survey the polluted level of total N-nitroso compounds (TNOC) in sausage on the market by the method established for the determination of TNOC in the sausage.
Methods:
The method of chemical fission-thermal energy analyzer was based on the previous method for the determination of TNOC in biological fluids which could distinguish between thermo-labile compounds and acid-labile compounds (TAC). Thirty-two samples of sausage were selected by a random sample procedure on the market. Chemical fission-thermal energy analyzer was used in the analysis of TNOC in the samples.
Results:
The results showed that nitrate from the sample was effectively removed by AG1-X8 anion exchange resin, and did not affected detection of N-nitroso compounds (NOC). 0.2% HCl/HAc was optimum concentration for TAC, and did not decompose unstable nitrosamides. Spiked with NOC mixture, the recovery in the sausage was 86% (83% -89%). The detection limit of this method was 6.6 microg(N-NO)/kg with 5.9% the relative standard deviation. TNOC detection rate and the average levels in the sausage were 93.8% and 49.09 microg/kg (95% Cl 39.15 - 126.35 microg/kg), respectively.
Conclusion:
This method may be veracity and could be repeated and could use for determination of TNOC in sausage.
