[Recommendation of a highly sensitive method for measuring hemoglobin in hemolytic test]

Area of Science:

• Biochemistry
• Clinical Chemistry
• Hematology

Context:

• The accurate determination of plasma hemoglobin (FHb) is crucial for diagnosing hemolytic conditions.
• Existing methods for FHb quantification may lack sensitivity or involve hazardous reagents.
• Viscacha hemolytic tests require a precise and safe analytical procedure.

Purpose:

• To present an optimized, highly sensitive, and reagent-safe method for plasma hemoglobin (FHb) determination.
• To validate the performance and safety of the improved 2,4-dichlorophenol (2,4-DCP) method for FHb analysis.
• To establish reference values for FHb in viscacha hemolytic tests.

Summary:

• An improved 2,4-dichlorophenol (2,4-DCP) method, based on the Trinder reaction, demonstrates 2.39 times higher sensitivity than the traditional phenol method for FHb.
• The 2,4-DCP method exhibits excellent run-to-run and day-to-day precision with stable reaction color, and is verified as non-carcinogenic and non-toxic.
• Sodium citrate is confirmed as an effective anticoagulant for preserving erythrocytes, and the established FHb reference range is 1-36.7 mg/L.

Impact:

• This optimized 2,4-DCP method provides a safer and more sensitive alternative for FHb quantification in viscacha hemolytic assays.
• The findings support the clinical and biomedical engineering application of this method for improved diagnostic accuracy.
• The reagent-safe nature of the 2,4-DCP method enhances laboratory safety and reduces environmental impact.