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Updated: Jul 12, 2026
15:04
Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Shift-excitation Raman difference spectroscopy-difference deconvolution method for the luminescence background
Iacopo Osticioli1, Angela Zoppi, Emilio Mario Castellucci
1University of Florence, Chemistry Department, Polo Scientifico Universitario, Sesto Fiorentino, Italy. iacopo.osticioli@.unifi.it
Applied Spectroscopy
|August 25, 2007
Summary
Shift-excitation Raman difference spectroscopy-difference deconvolution (SERDS-DDM) effectively suppresses fluorescence in solid samples. This method reconstructs pure Raman spectra, improving weak band detection compared to baseline subtraction.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Materials Science
Background:
- Fluorescence interference complicates Raman spectroscopy of solid samples.
- Accurate Raman spectra are crucial for material identification and analysis.
- Existing fluorescence suppression methods have limitations.
Purpose of the Study:
- To evaluate the efficacy of the shift-excitation Raman difference spectroscopy-difference deconvolution (SERDS-DDM) method for fluorescence suppression.
- To demonstrate SERDS-DDM's ability to reconstruct pure Raman spectra from samples with intrinsic or external fluorescence.
- To compare SERDS-DDM performance against traditional multi-point baseline subtraction techniques.
Main Methods:
- Utilized a tunable diode laser at 684 nm with a micro-Raman apparatus.
- Employed a monochromator for excitation frequency stability monitoring.
- Applied mathematical deconvolution to SERDS data for fluorescence removal.
Main Results:
- Successfully reconstructed the Raman spectrum of cinnabar pigment with intrinsic fluorescence.
- Isolated the Raman spectrum of a pure sulfur crystal from external luminescence background.
- Demonstrated significant improvement in weak band detection using SERDS-DDM over baseline subtraction.
Conclusions:
- SERDS-DDM is a feasible and effective method for fluorescence suppression in Raman spectroscopy of solid samples.
- The technique offers enhanced sensitivity for detecting weak Raman signals.
- Practical guidelines for implementing SERDS-DDM are provided.
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