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Investigation on sensitized chemiluminescence systems and their mechanism for five fluoroquinolones

Hanwen Sun1, Liqing Li, Xueyan Chen

  • 1College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Baoding 071002, China. hanwen@mail.hbu.cn

Analytica Chimica Acta
|August 29, 2007
PubMed

Insights

Novel chemiluminescence (CL) systems were developed for five fluoroquinolones: lomefloxacin (LMFX), ofloxacin (OFLX), norfloxacin (NFLX), gatifloxacin (GAFX), and enoxacin (ENX). These enhanced CL methods offer sensitive and precise residue analysis in food and biological samples.

Area of Science:

  • Analytical Chemistry
  • Chemiluminescence Spectroscopy
  • Fluoroquinolone Analysis

Background:

  • Fluoroquinolones are widely used antibiotics, necessitating reliable methods for detecting their residues.
  • Existing analytical techniques may lack the sensitivity or efficiency required for trace-level detection.
  • Chemiluminescence (CL) offers a sensitive detection pathway but requires optimized reaction systems for specific analytes.

Purpose of the Study:

  • To establish and investigate novel chemiluminescence reaction systems for five fluoroquinolones.
  • To elucidate the sensitized CL emission mechanisms for these fluoroquinolone systems.
  • To evaluate the potential of these enhanced CL systems for residue analysis in complex matrices.

Main Methods:

  • Development of five distinct chemiluminescence systems using Ce(IV) and various co-reactants (S2O3(2-), S2O4(2-)) with specific fluoroquinolones.
  • Investigation of CL emission mechanisms through comparison with fluorescence spectra and energy transfer pathways.
  • Optimization of CL reaction conditions to maximize sensitivity and precision.

Main Results:

  • Successful establishment of five enhanced CL systems for lomefloxacin, ofloxacin, norfloxacin, gatifloxacin, and enoxacin.
  • Elucidation of energy transfer mechanisms, including intermolecular transfer from SO2* and intramolecular transfer to Eu3+ or Dy3+ in specific systems.
  • Demonstrated good linearity, high sensitivity, and precision for the developed CL methods.

Conclusions:

  • The proposed five enhanced chemiluminescence systems provide sensitive and precise analytical methods for fluoroquinolones.
  • The mechanistic studies reveal key energy transfer processes enhancing CL emission.
  • These methods show significant potential for the accurate residue analysis of target fluoroquinolones in food and biological samples.

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