High sensitivity detection of bisphenol A using liposome chromatography

Area of Science:

• Analytical Chemistry
• Environmental Science
• Biochemistry

Background:

• Bisphenol A (BPA) is a common endocrine disruptor with potential health risks.
• Sensitive and reliable detection methods for BPA are crucial for environmental monitoring and public health.
• Existing methods like ELISA have limitations in detection range and sample applicability.

Purpose of the Study:

• To develop a highly sensitive and efficient analytical method for detecting bisphenol A (BPA).
• To create a novel detection system combining antibody-based affinity chromatography and enzyme-triggered liposome-based signal amplification.
• To evaluate the performance of the developed system for BPA detection in environmental samples.

Main Methods:

• Immobilization of anti-BPA antibody on a protein G column for oriented capture.
• Conjugation of a BPA derivative to phospholipase A2 (PLA2) for signal generation.
• Competitive binding assay on the antibody column followed by detection on a calcein-entrapped liposome column.
• Signal amplification via PLA2-catalyzed hydrolysis of liposomes, releasing fluorescent dye.

Main Results:

• The developed antibody-liposome system achieved a wide BPA detection range of 0.02-140 ng mL(-1).
• This detection range is significantly broader than that obtained by competitive ELISA (0.03-6.6 ng mL(-1)).
• The method demonstrated applicability to environmental samples, detecting BPA in river water (0.1 ng mL(-1)) and soil extracts (10 ng g(-1)).

Conclusions:

• The tandem antibody-liposome column system provides a highly sensitive and effective method for BPA detection.
• The system offers advantages in detection range and adaptability to real-world environmental samples.
• The method's compatibility with HPLC suggests potential for online monitoring applications.