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Updated: Jul 12, 2026

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
Flow-injection post chemiluminescence determination of atropine sulfate
1School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, People's Republic of China; Jilin Medical College, Jilin 132013, People's Republic of China.
Abstract:
A new post chemiluminescence (PCL) reaction was observed when atropine sulfate was injected into the reaction mixture after the finish of CL reaction of Ce(IV) and sodium sulfite. The possible mechanism for the PCL reaction was discussed via the investigation of the CL kinetic characteristics, the CL spectra, the UV absorption spectra and the fluorescence spectra of some related substances. The flow injection PCL method for the determination of atropine sulfate was established. The relative standard deviation (R.S.D.) was 2.8% (n=11, c=5.0 x 10(-6)g mL(-1)). The PCL intensity responded linearly to the concentration of atropine sulfate in the range 1.0 x 10(-6) to 5.0 x 10(-5)g mL(-1) with a linear correlation of 0.9947. The detection limit was 4 x 10(-7)g mL(-1) atropine sulfate. The method had been applied to the determination of atropine sulfate in the tablets and the results were consistent with the method of Chinese pharmacopoeia.
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