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Structural analysis of the decanucleotide duplex d(GGTAATTACC)2 using 1H NMR spectroscopy
K J Embrey1, M S Searle, D J Craik
1Victorian College of Pharmacy, School of Pharmaceutical Chemistry, Parkville, Vic., Australia.
Biochemistry International
|June 1, 1991
Abstract:
The conformation of the decanucleotide duplex d(GGTAATTACC)2 has been investigated in solution by one- and two-dimensional proton NMR spectroscopy. Intra- and inter-nucleotide two-dimensional nuclear Overhauser enhancement data, recorded at mixing times between 15 and 250 ms, reveal a right-handed B-DNA structure. The data also show that the A-T basepairs of the TAATTA tract are highly propeller twisted and the minor groove is particularly narrow.