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Updated: Jul 12, 2026

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

Published on: November 1, 2018

Podocyte antigens and glomerular disease.

Pierre Ronco¹, Hanna Debiec

¹INSERM Unit UMR S 702, Pierre et Marie Curie University-Paris 6, AP-HP (Tenon Hospital), Paris, France. pierre.ronco@tnn.aphp.fr

Nephron. Experimental Nephrology

|August 30, 2007

Summary

Researchers identified neutral endopeptidase (NEP) as a key podocyte antigen in membranous nephropathy (MN). This discovery may pave the way for targeted immunotherapies for this kidney disease.

Area of Science:

Nephrology
Immunology
Molecular Biology

Background:

Membranous nephropathy (MN) is a leading cause of adult nephrotic syndrome.
It is an immune-mediated kidney disease involving subepithelial immune deposits and podocyte injury.
Current MN treatments are empirical, lacking targeted therapies.

Purpose of the Study:

To identify target antigens of circulating antibodies in membranous nephropathy (MN).
To investigate the role of these antigens in disease pathogenesis.
To explore potential for antigen-specific immunotherapy.

Main Methods:

Analysis of antibody specificity and glomerular deposits in a rat model (Heymann nephritis) and human MN patients.
Utilized knowledge of megalin as a target antigen in the rat model.

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An Efficient Sieving Method to Isolate Intact Glomeruli from Adult Rat Kidney

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Glomerular Outgrowth as an Ex Vivo Assay to Analyze Pathways Involved in Parietal Epithelial Cell Activation

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Isolation of Glomeruli and In Vivo Labeling of Glomerular Cell Surface Proteins

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Investigated the human counterpart of megalin.

Main Results:

Identified neutral endopeptidase (NEP) as the human podocyte target antigen in MN.
Confirmed that antibodies against megalin or NEP trigger subepithelial immune deposits.
Observed complement activation, specifically C5b-9 formation, in response to these antibodies.

Conclusions:

Podocyte membrane antigens are likely involved in idiopathic MN.
Identifying these antigens and immunodominant epitopes is crucial.
This knowledge may enable the development of specific immunotherapies to induce tolerance.