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[Studies on the extraction of active polysaccharide from Dendrobium huoshanense with Box-Behnken method]
Sen Huang1, Xue-Qiang Zha, Jian-Ping Luo
1School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China.
Objective:
In order to obtain preferable extraction rate of polysaccharide from Dendrobium huoshanense, best extraction conditions and decoloration method were investigated.
Methods:
Five influential factors including extraction temperature, extraction time, ratio of material to water, repetition times and extraction pH were carried out. Extraction model of polysaccharide from Dendrobium huoshanense was established by Box-Behnken method.
Results:
In the conditions of 69.4 degrees C of the temperature, 1.57h of the extraction time, 1: 20 of the ratio of material to water, two of the repetition times and 5.9 of the pH, the extraction rate reached the peak of 0.1821 g/g. Taking into account of decoloration, 65.47% of decoloration rate was obtained with DEAE-52 method.
Conclusion:
Extraction model of polysaccharide from Dendrobium huoshanense established by Box-Behnken method can precisely predict the experiment results. In the best conditions deduced from the model, the extraction rate of polysccharide is high and so is the decoloration rate.
