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Chromosome 17- and p53 changes in lymphoma
M A Rodriguez1, R J Ford, A Goodacre
1Department of Hematology, University of Texas M.D. Anderson Cancer Center, Houston 77030.
British Journal of Haematology
|December 1, 1991
Summary
Changes to chromosome 17 short arm (17p), particularly involving the p53 gene, are linked to aggressive non-Hodgkin
Area of Science:
- Genetics
- Oncology
- Molecular Biology
Background:
- Chromosome 17 short arm (17p) abnormalities are associated with poor prognosis in lymphoma.
- The tumor suppressor gene p53 is located on 17p.
Purpose of the Study:
- To investigate genomic alterations of the p53 gene in lymphoma cell lines and patient samples.
- To correlate 17p changes with clinical outcomes in non-Hodgkin's lymphoma.
Main Methods:
- Southern blot analysis was used to detect p53 gene alterations.
- Cytogenetic analysis identified chromosomal abnormalities in lymphoma samples.
Main Results:
- Four cell lines and four tumor samples exhibited numerical or structural abnormalities of 17p.
- Allelic loss of the p53 gene was detected in two cell lines and one tumor.
- One cell line showed rearrangement within the p53 gene.
Conclusions:
- Genomic alterations of the p53 gene on chromosome 17p may contribute to the aggressive progression of non-Hodgkin's lymphoma.
- These findings suggest p53 gene status as a potential prognostic marker.