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Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
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Flexibility in targeting and insertion during bacterial membrane protein biogenesis

Edwin van Bloois1, Corinne M ten Hagen-Jongman, Joen Luirink

  • 1Department of Molecular Microbiology, Institute of Molecular Cell Biology, Vrije Universiteit, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.

Biochemical and Biophysical Research Communications
|August 31, 2007
PubMed
Summary

Escherichia coli inner membrane protein biogenesis uses targeting factors like signal recognition particle (SRP) and Sec-translocon. Engineered proteins reveal these factors are used facultatively based on structural features.

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