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[Synthesis of delta virus cDNA of China Sichuan strain]
1Research Unit of Viral Hepatitis, Institute of Biochemistry and Molecular Biology.
Hua Xi Yi Ke Da Xue Xue Bao = Journal of West China University of Medical Sciences = Huaxi Yike Daxue Xuebao
|March 1, 1991
Abstract:
delta virus cDNA of China Sichuan strain, 1.2 kb in length, was synthesized by extraction of RNA from liver tissue in a patient with subacute severe hepatitis, followed by reverse transcription and polymerase chain reaction (RT-PCR). The result was confirmed by Southern blot hybridization using 32P and biotin labelled delta virus cDNA (HN88, cloned in USA) probes respectively.