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Related Experiment Video

Updated: Jul 12, 2026

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Asymmetrical Flow Field-Flow Fractionation for Sizing of Gold Nanoparticles in Suspension

Published on: September 11, 2020

Sedimentation field flow fractionation: applications.

J J Kirkland, W W Yau

Science (New York, N.Y.)

|October 8, 1982

Summary

This summary is machine-generated.

Sedimentation field flow fractionation (SFFF) offers high-resolution separation for biological macromolecules and particles. This advanced technique achieves superior resolution in less time compared to traditional centrifugation methods.

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Area of Science:

Analytical Chemistry
Biophysics
Materials Science

Background:

Sedimentation field flow fractionation (SFFF) is an emerging separation technique.
Conventional centrifugation methods have limitations in resolution and time efficiency.

Purpose of the Study:

To highlight the capabilities of SFFF for separating biological macromolecules, colloids, and particulates.
To compare the performance of SFFF with conventional centrifugation.

Main Methods:

Utilizing advanced instrumentation for SFFF operation.
Achieving high rotor speeds up to 32,000 rpm (approx. 85,000 g).

Main Results:

SFFF enables high-resolution separation of diverse materials, including those with molecular weights as low as 5 x 10^5.
The method demonstrates superior resolution compared to centrifugation.
SFFF achieves these separations in significantly shorter timeframes.

Conclusions:

SFFF is a powerful and efficient method for separating a wide range of biological and particulate materials.
The technique offers advantages in resolution and speed over conventional methods.
Advances in instrumentation enhance the applicability of SFFF for complex samples.