Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RENEB Inter-Laboratory Comparison 2021: The Gene Expression Assay.

Radiation research·2023
Same author

Outbreak of Pseudomonas aeruginosa producing VIM carbapenemase in an intensive care unit and its termination by implementation of waterless patient care.

Critical care (London, England)·2021
Same author

Exclusive Magnetic Excitation Enabled by Structured Light Illumination in a Nanoscale Mie Resonator.

ACS nano·2018
Same author

Heat-resistant, extended-spectrum β-lactamase-producing Klebsiella pneumoniae in endoscope-mediated outbreak.

The Journal of hospital infection·2016
Same author

Towards a full retrieval of the deformation tensor F using convergent beam electron diffraction.

Ultramicroscopy·2015
Same author

USP29 controls the stability of checkpoint adaptor Claspin by deubiquitination.

Oncogene·2014

Related Experiment Video

Updated: Jul 11, 2026

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
14:58

Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

Published on: June 2, 2010

Scanning interferometric apertureless microscopy: optical imaging at 10 angstrom resolution.

F Zenhausern, Y Martin, H K Wickramasinghe

    Science (New York, N.Y.)
    |August 25, 1995
    PubMed
    Summary

    Near-field optical microscopy now achieves 10 angstrom resolution using interferometry. This technique measures scattered electric fields via probe tip vibrations, enabling unprecedented nanoscale imaging.

    More Related Videos

    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
    08:13

    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

    Published on: April 8, 2019

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    Related Experiment Videos

    Last Updated: Jul 11, 2026

    Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters
    14:58

    Optical Scatter Microscopy Based on Two-Dimensional Gabor Filters

    Published on: June 2, 2010

    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
    08:13

    A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

    Published on: April 8, 2019

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
    14:09

    High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip

    Published on: November 16, 2019

    Area of Science:

    • Physics
    • Nanotechnology
    • Optical Microscopy

    Background:

    • Conventional near-field optical microscopy relies on dipole-sample polarizability interactions.
    • Achieving sub-nanometer resolution in optical microscopy has been a long-standing challenge.

    Purpose of the Study:

    • To demonstrate a novel interferometric near-field optical microscopy technique.
    • To achieve an unprecedented resolution of 10 angstroms.

    Main Methods:

    • Utilizing an interferometer to measure scattered electric field variations.
    • Encoding these variations as optical phase modulation using a vibrating probe tip.
    • Employing a new contrast mechanism based on modulated dipole-dipole coupling.

    Main Results:

    • Demonstrated interferometric near-field optical microscopy with 10 angstrom resolution.
    • Successfully measured scattered electric field variations through optical phase modulation.
    • Validated a novel contrast mechanism based on sensing modulated dipole-dipole coupling.

    Conclusions:

    • Interferometric near-field optical microscopy offers a pathway to ultra-high resolution imaging.
    • The demonstrated technique overcomes limitations of traditional near-field methods.
    • This advancement has significant implications for nanoscale surface analysis.