Related Experiment Video
Updated: Jul 12, 2026

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
Published on: September 26, 2025
[Expression and biological activities of arresten in CHO cells]
Miao-yun Long1, Qi-chang Zheng, Zi-fang Song
1Department of Hepatobiliary Surgery, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China. lmy1023@sohu.com
Objective:
To explore the eukaryotic expression of arresten in CHO cells and to investigate its basic biological activities.
Methods:
CHO cells were divided into three groups: transfected pSecTag-arresten group, transfected pSecTag group and control group without transfection. PSecTag-arresten was transfected into CHO cells by Lipofectamine 2000 method. The arresten mRNA in CHO cells was assayed by RT-PCR. The protein expression of arresten gene was examined by Western-Blot. The cells expressing arresten were screened out by Zeocin. The effect of arresten on huvec cell migration and anchoring to three-dimensional vascular structures was measured.
Results:
The result of RT-PCR and Western-blot showed that arresten gene has been successfully transfected into CHO cells and expressed in those cells. Arrssten inhibited huvec cell migration and anchoring to three-dimensional vascular structures.
Conclusion:
CHO cells expressing arresten have been obtained successfully. Arresten can inhibit huvec cell migration and anchoring to three-dimensional vascular structures, indicating that it might be one of its anti-angiogenetic approaches.
Related Concept Videos
Negative Regulator Molecules
Cell Specific Gene Expression
Amplifying Signals via Enzymatic Cascade
Eukaryotic Transcription Inhibitors
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
DNA Damage can Stall the Cell Cycle
DNA Damage Can Stall the Cell Cycle

