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Updated: Jul 12, 2026

Modeling Neuronal Death and Degeneration in Mouse Primary Cerebellar Granule Neurons
Published on: November 6, 2017
Formation of archegonium chamber is associated with nucellar-cell programmed cell death in Ginkgo biloba
Da-Hui Li1, Xiong Yang, Ke-Ming Cui
1School of Pharmaceutical Sciences, Peking University, Beijing. dhl@water.pku.edu.cn
Abstract:
The archegonium chamber in Ginkgo biloba L. is a pathway for spermatozoids swimming towards the archegonium for fertilization. The objective of this study was to elucidate the mechanism of archegonium chamber formation. The terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and DNA ladder demonstrated that the nucellar cell death, coordinated with the archegonium chamber formation, was a process of programmed cell death. Cytochemical localization of Ca(2+) in these nucellar cells was determined by means of in situ precipitation with potassium pyroantimonate and electron microscopic visualization, in order to study the relation between Ca(2+) and programmed cell death. The results showed an early uptake of the mitochondrial calcium particles in the nucellar cells undergoing programmed cell death. Together with other dynamic changes in Ca(2+) subcellular distribution, this indicates that Ca(2+) may play a role in the regulation of mitochondria-mediated programmed events in the nucellar cells.
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