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c-Myc interacts with TRF1/PIN2 and regulates telomere length
1Department of Therapeutic Radiology, Yale University School of Medicine, P.O. Box 208040, New Haven, CT 06520, USA.
Biochemical and Biophysical Research Communications
|September 4, 2007
Summary
The transcriptional activator c-Myc directly binds to TRF1/PIN2, a protein that regulates telomere length. This interaction, mediated by c-Myc's bHLH domain, suggests c-Myc plays a role in telomere maintenance and genomic stability.
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Telomeres protect chromosome ends and maintain genomic stability.
- TRF1/PIN2 regulates telomere length by capping and blocking telomerase access.
Purpose of the Study:
- To elucidate the mechanism by which TRF1/PIN2 regulates telomere length.
- To identify proteins interacting with TRF1/PIN2.
Main Methods:
- Yeast two-hybrid screening to identify binding partners.
- In vitro and in vivo assays to confirm protein interactions.
- Overexpression studies to assess functional impact.
Main Results:
- The transcriptional activator c-Myc was identified as a TRF1/PIN2-binding protein.
- The interaction between c-Myc and TRF1/PIN2 was confirmed both in vitro and in vivo.
- Overexpression of c-Myc's bHLH domain, which mediates binding to TRF1/PIN2, resulted in telomere elongation.
Conclusions:
- c-Myc directly binds to TRF1/PIN2.
- c-Myc may regulate telomere length through its interaction with TRF1/PIN2.
- This interaction potentially links c-Myc's transcriptional role to telomere maintenance and genomic stability.
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