Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peptide signals encode protein localization.

Jay H Russell1, Kenneth C Keiler

  • 1Department of Biochemistry and Molecular Biology, The Pennsylvania State University, 401 Althouse, University Park, PA 16827, USA.

Journal of Bacteriology
|September 4, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>trans</i>-Translation inhibitors that kill <i>Mycobacterium tuberculosis</i> and pathogenic non-tuberculous mycobacteria also disrupt metal homeostasis.

Microbiology (Reading, England)·2026
Same author

Ribosomal Protein bL27 Protects Translating Ribosomes from tmRNA-SmpB.

bioRxiv : the preprint server for biology·2026
Same author

<i>trans</i>-Translation inhibitors that kill <i>M. tuberculosis</i> and pathogenic Non-tuberculous Mycobacteria have a dual mechanism of action.

bioRxiv : the preprint server for biology·2026
Same author

Targeting Recoding by <i>trans</i>-Translation to Develop Antibiotics.

Cold Spring Harbor perspectives in biology·2025
Same author

A <i>trans</i>-translation inhibitor that targets ribosomal protein bL12 kills <i>Mycobacterium tuberculosis</i>.

Journal of bacteriology·2025
Same author

A <i>trans</i> -translation inhibitor kills <i>Mycobacterium tuberculosis</i> by targeting ribosomal protein bL12.

bioRxiv : the preprint server for biology·2025
Same journal

The bacterial SOS response promotes the expression of the transposase encoded by IS<i>CR</i> mobile genetic elements.

Journal of bacteriology·2026
Same journal

Development of a gene-editing strategy to overcome genetic intractability in <i>Lactobacillus johnsonii</i>.

Journal of bacteriology·2026
Same journal

Bactofilins are essential spatial organizers of peptidoglycan insertion in the Lyme disease spirochete <i>Borrelia burgdorferi</i>.

Journal of bacteriology·2026
Same journal

DNA damage-associated vesicle production in <i>Stenotrophomonas maltophilia</i> is mediated by the maltocin endolysin.

Journal of bacteriology·2026
Same journal

Characterization of <i>Helicobacter pylori</i> aggregation reveals a requirement for both AlpA and AlpB.

Journal of bacteriology·2026
Same journal

Cross-regulation of amino acid synthesis and anaerobic electron transfer by MetR-mediated methionine signaling.

Journal of bacteriology·2026
See all related articles

Researchers discovered short peptide sequences that can direct bacterial protein localization. This finding suggests protein location within cells may be predictable using bioinformatics, moving beyond complex protein interactions.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Cell Biology

Background:

  • Precise intracellular localization of bacterial proteins is crucial for cellular function.
  • Mechanisms for encoding protein localization information within bacterial cells are largely unknown.
  • Current understanding often relies on complex protein-protein interactions.

Purpose of the Study:

  • To identify novel mechanisms for bacterial protein localization.
  • To investigate if short peptide sequences can encode localization information.
  • To explore the potential for predicting protein cellular location using bioinformatics.

Main Methods:

  • Screening of peptide libraries fused to green fluorescent protein (GFP).
  • Identification of specific peptide sequences influencing protein localization.

Related Experiment Videos

  • Analysis of peptide-directed localization to cellular structures like helical filaments or midcell.

    • Main Results:

    • Discovery of 20-amino-acid peptides capable of directing protein localization.
    • Demonstration that these peptides can target proteins to specific cellular sites.
    • Evidence that localization information can be encoded in short peptide motifs.

    Conclusions:

    • Short peptide sequences are sufficient to encode bacterial protein localization information.
    • This challenges the notion that only complex interactions dictate protein location.
    • Suggests potential for bioinformatic prediction of protein intracellular destinations.