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Updated: Jul 12, 2026
Water in Oil Emulsions: A New System for Assembling Water-soluble Chlorophyll-binding Proteins with Hydrophobic Pigments
Published on: March 21, 2016
Purification, crystallization and preliminary X-ray diffraction analysis of water-soluble chlorophyll-binding protein
Takayuki Ohtsuki1, Shigeru Ohshima, Akira Uchida
1Department of Bimolecular Science, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
Abstract:
A water-soluble chlorophyll-binding protein (WSCP) with photoconvertibility from Chenopodium album was extracted, purified and crystallized in a darkroom. Green crystals suitable for data collection appeared in about 10 d. A native data set was collected to 2.0 A resolution at 100 K. The space group of the crystal was determined to be orthorhombic I222 or I2(1)2(1)2(1), with unit-cell parameters a = 48.13, b = 60.59, c = 107.21 A. Preliminary analysis of the X-ray data indicated that there is one molecule per asymmetric unit.
