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In vivo Imaging Method to Distinguish Acute and Chronic Inflammation
Published on: August 16, 2013
Low-level laser therapy (GaAs lambda = 904 nm) reduces inflammatory cell migration in mice with
Fernanda Correa1, Rodrigo Alvaro Brandão Lopes Martins, Joao Carlos Correa
1University Nove Julho, São Paulo, Brazil.
Objective:
This study was designed to study the effect of an infrared low-level laser (GaAs lambda = 904 nm, 4 mW) on inflammatory cell migration in lipopolysaccharide (LPS)-induced peritonitis in mice.
Background Data:
It has been suggested that red wavelengths of low-level laser therapy (LLLT) can exert anti-inflammatory effects, but little is known about the anti-inflammatory effects of infrared lasers. Peritonitis is a potentially life-threatening inflammatory condition that may be suitable for studying anti-inflammatory effects of infrared lasers.
Methods:
Sixty male mice were randomly divided into five groups, and one group was given an intraperitoneal sterile saline injection. In the remaining four groups, peritonitis was induced by an intraperitoneal LPS injection. Animals in three of the LPS groups were irradiated at a single point over the peritoneum with doses of 3 J/cm(2), 7.5 J/cm(2), and 15 J/cm(2), respectively. The fourth group injected with LPS was an LPS-control group.
Results:
At 6 hours after injection the groups irradiated with doses of 3 J/cm(2) and 7.5 J/cm(2) had a reduced number of neutrophil cells in the peritoneal cavity compared with the LPS-control group, and there were significant differences between the number of neutrophils in the peritoneal cavity between the LPS-control group and groups irradiated with doses of 3 J/cm(2) (-42%) and 7.5 J/cm(2) (-70%). In the group irradiated with 15 J/cm(2), neutrophil cell counts were lower than, but not significantly different from, LPS controls (-38%; p = 0.07). At 24 hours after injection, both neutrophil and total leukocyte cell counts were lower in all the irradiated groups than in the LPS controls. The 3-J/cm(2) exposure group showed the best results at 24 hours, with reductions of 77% in neutrophil and 49% in leukocyte counts.
Conclusion:
Low-level laser therapy (904 nm) can reduce inflammatory cell migration in mice with LPS-induced peritonitis in a dose-dependent manner.
Insights
Infrared low-level laser therapy (LLLT) effectively reduced inflammatory cell migration in mice with LPS-induced peritonitis. This dose-dependent effect shows promise for treating inflammatory conditions.
Area of Science:
- Biomedical Engineering
- Laser Physics
- Immunology
Background:
- Low-level laser therapy (LLLT) with red wavelengths is known for anti-inflammatory effects.
- The anti-inflammatory potential of infrared lasers remains less explored.
- Peritonitis, an inflammatory condition, serves as a model for evaluating infrared laser efficacy.
Purpose of the Study:
- To investigate the anti-inflammatory effects of infrared low-level laser (904 nm) on inflammatory cell migration.
- To assess the dose-dependent response of infrared LLLT in a mouse model of lipopolysaccharide (LPS)-induced peritonitis.
Main Methods:
- Sixty male mice were used, with peritonitis induced by intraperitoneal LPS injection in most groups.
- Experimental groups received infrared laser irradiation (904 nm) at varying doses (3, 7.5, 15 J/cm²).
- A control group received sterile saline, and another received LPS without laser treatment.
Main Results:
- Irradiation with 3 J/cm² and 7.5 J/cm² significantly reduced neutrophil migration at 6 hours post-LPS injection.
- At 24 hours, all irradiated groups showed reduced neutrophil and total leukocyte counts compared to controls.
- The 3 J/cm² dose demonstrated the most significant reduction in inflammatory cells (77% neutrophils, 49% leukocytes).
Conclusions:
- Infrared low-level laser therapy (904 nm) effectively reduces inflammatory cell migration in LPS-induced peritonitis.
- The anti-inflammatory effect is dose-dependent, with lower doses showing significant efficacy.
- Infrared LLLT presents a potential therapeutic strategy for managing inflammatory conditions like peritonitis.
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