Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Irradiation effect on aging red blood cells.

E K Jeter1, R H Gadsden, J C Cate

  • 1Department of Pathology/Laboratory Medicine, Medical University of South Carolina, Charleston 29425-0701.

Annals of Clinical and Laboratory Science
|November 11, 1991
PubMed
Summary

Irradiating stored red blood cells (RBC) causes potassium leakage, especially in older cells. Storage duration before irradiation impacts how quickly potassium equilibrium is reached, but not other measures like free hemoglobin.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation and implementation of the gel test for indirect antiglobulin testing in a community hospital laboratory.

Archives of pathology & laboratory medicine·1999
Same author

Two sensitive immunometric assays for serum thyroid stimulating hormone evaluated.

Annals of clinical and laboratory science·1998
Same author

The stability of ethanol in human whole blood controls: an interlaboratory evaluation.

Journal of analytical toxicology·1997
Same author

Cytophilic immunoglobulin G binding on neutrophils from a child with malignant osteopetrosis who developed fatal acute respiratory distress mimicking transfusion-related acute lung injury.

American journal of hematology·1996
Same author

Regulation of brain m calpain Ca2+ sensitivity by mixtures of membrane lipids: activation at intracellular Ca2+ level.

Journal of neuroscience research·1996
Same author

Identification and quantification of hemoglobins A, F, S, and C by automated chromatography.

Clinical chemistry·1996

Area of Science:

  • Blood banking and transfusion medicine
  • Cellular therapy and preservation

Background:

  • Irradiation of red blood cells (RBC) is crucial for immunocompromised patients.
  • Previously irradiated RBCs require extended storage, necessitating studies on irradiation's impact on aged cells.

Purpose of the Study:

  • To investigate the effect of gamma irradiation on aging stored red blood cells (RBC).
  • To determine how storage duration before irradiation influences RBC characteristics post-irradiation.

Main Methods:

  • Stored RBC units (AS-1) of varying ages (10-40 days) were divided and one unit of each pair was irradiated (2,000 rads).
  • Measurements included extracellular potassium (K+), plasma free hemoglobin (PFH), lactate dehydrogenase (LD), and 2,3-diphosphoglycerate (2,3-DPG) activity over time.

Related Experiment Videos

Main Results:

  • All irradiated RBC units reached potassium equilibrium (60-70 mmol/L), with older cells requiring less time.
  • RBC age at irradiation inversely correlated with the time to reach potassium equilibrium.
  • No significant effect of storage duration prior to irradiation was observed on PFH, LD, or 2,3-DPG levels.

Conclusions:

  • Irradiation of stored RBCs leads to increased potassium leakage, influenced by RBC age.
  • Further in vivo studies are needed to establish safe post-irradiation storage limits for RBCs.
  • Storage duration before irradiation does not affect key biochemical markers like PFH, LD, or 2,3-DPG.