Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
The Replisome03:01

The Replisome

DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with the...
DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vitrification-based tissue-to-organoid cryopreservation workflow for colorectal cancer.

New biotechnology·2026
Same author

Natural antisense transcript Nat9a suppresses Scn9a (Na<sub>V</sub>1.7) expression in parvalbumin-positive proprioceptive and inhibitory neurons.

Scientific reports·2026
Same author

Neutralization of SARS-CoV-2 by IgM-14 via engagement of two distinct spike epitopes.

PLoS pathogens·2026
Same author

CAMPER: mechanistic artificial intelligence for designing peptides that target MRSA persisters.

Nature communications·2026
Same author

Biophysical characterisation of mRNA structure and its impact on integrity analysis by liquid chromatography and capillary gel electrophoresis methods.

Journal of chromatography. A·2026
Same author

Analysis of mRNA multimerisation (aggregation) using non-denaturing ion-pair reversed-phase liquid chromatography.

Journal of chromatography. A·2026

Related Experiment Video

Updated: Jul 11, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Hexameric ring structure of human MCM10 DNA replication factor.

Andrei L Okorokov1, Alastair Waugh, Julie Hodgkinson

  • 1Department of Pathology, University College London, London WC1E 6JJ, UK.

EMBO Reports
|September 8, 2007
PubMed
Summary
This summary is machine-generated.

Minichromosome maintenance 10 (MCM10) is vital for DNA replication. Its newly discovered ring-shaped hexamer structure reveals how it links key replication proteins, facilitating DNA synthesis.

More Related Videos

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Related Experiment Videos

Last Updated: Jul 11, 2026

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG
10:11

Hybrid Ensemble and Single-molecule Assay to Image the Motion of Fully Reconstituted CMG

Published on: July 26, 2024

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy
11:40

Preparation of the Mgm101 Recombination Protein by MBP-based Tagging Strategy

Published on: June 25, 2013

Area of Science:

  • Molecular biology
  • Biochemistry
  • Structural biology

Background:

  • Minichromosome maintenance 10 (MCM10) is a crucial protein for eukaryotic DNA replication.
  • MCM10 acts as a physical linker between the MCM2-7 helicase and DNA polymerases during origin firing.

Purpose of the Study:

  • To determine the molecular structure of human MCM10.
  • To elucidate the structural basis for MCM10's function in DNA replication.

Main Methods:

  • Electron microscopy
  • Single-particle analysis
  • Biochemical assays

Main Results:

  • The molecular structure of human MCM10 was determined.
  • MCM10 forms a ring-shaped hexamer.
  • The hexamer possesses large central and smaller lateral channels, along with inner chambers.

Conclusions:

  • The MCM10 structure provides a docking module for assembling the eukaryotic DNA replication machinery.
  • This structural insight explains MCM10's critical role in facilitating DNA replication initiation.