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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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Related Experiment Video

Updated: Jul 11, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

Micro-RNA profiling in kidney and bladder cancers.

Fedra Gottardo¹, Chang Gong Liu, Manuela Ferracin

¹Department of Urology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA.

Urologic Oncology

|September 11, 2007

Summary

This summary is machine-generated.

Micro-RNAs are deregulated in kidney and bladder cancers, indicating their role in tumor development. Specific micro-RNAs were identified as significantly up-regulated in these cancers, suggesting potential diagnostic markers.

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Last Updated: Jul 11, 2026

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Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Micro-RNAs (miRNAs) are small noncoding RNAs that regulate gene expression.
Aberrant miRNA expression is implicated in various cancers.
Understanding miRNA dysregulation in kidney and bladder cancers is crucial.

Purpose of the Study:

To investigate the expression profiles of 245 micro-RNAs in human kidney and bladder tumors.
To identify specific micro-RNAs associated with kidney and bladder cancer.
To explore the potential role of micro-RNAs in cancer development and progression.

Main Methods:

Analysis of 27 kidney specimens (20 carcinomas, 4 benign tumors, 3 normal parenchyma) and 27 bladder specimens (25 carcinomas, 2 normal mucosa).
Micro-RNA profiling using an oligonucleotide microchip containing 368 probes for 245 human and mouse micro-RNA genes.
Hybridization of total RNA extracted from tumor and normal tissues.

Main Results:

Four micro-RNAs (miR-28, miR-185, miR-27, let-7f-2) were significantly up-regulated in renal cell carcinoma compared to normal kidney tissue.
Ten micro-RNAs (miR-223, miR-26b, miR-221, miR-103-1, miR-185, miR-23b, miR-203, miR-17-5p, miR-23a, miR-205) were significantly up-regulated in bladder cancers compared to normal bladder mucosa.
No differential micro-RNA expression was observed across different stages of kidney cancer. miR-26b showed a decreasing trend with increasing tumor stage in bladder cancer.

Conclusions:

Distinct micro-RNAs are deregulated in kidney and bladder cancers, suggesting their involvement in tumorigenesis.
These findings highlight the potential of micro-RNAs in the development and progression of these malignancies.
Further research is warranted to elucidate the precise role of micro-RNAs and their utility as diagnostic and prognostic tools.