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Development of a screening method for five sulfonamides in salmon muscle tissue using thin-layer chromatography
1CanTest Ltd., Vancouver, British Columbia, Canada.
Journal of Chromatography
|August 30, 1991
Summary
A new thin-layer chromatographic method accurately analyzes five sulfonamides in salmon. This technique, using matrix solid-phase dispersion, offers sensitive detection crucial for food safety analysis.
Area of Science:
- Analytical Chemistry
- Food Science
- Pharmacology
Background:
- Sulfonamides are widely used veterinary drugs, necessitating reliable methods for their detection in food products.
- Residue analysis in complex matrices like fish muscle is challenging due to potential interferences.
- Accurate quantification of sulfonamide residues ensures compliance with food safety regulations and protects public health.
Purpose of the Study:
- To develop and validate a sensitive thin-layer chromatographic (TLC) method for analyzing five specific sulfonamides in salmon muscle tissue.
- To establish method detection limits and recovery rates for sulfonamide quantification.
- To assess the linearity and applicability of the developed method for residue monitoring.
Main Methods:
- Matrix solid-phase dispersion (MSPD) was employed for sample preparation, grinding salmon tissue with C18-derivatized silica gel.
- Extraction and cleanup were performed using a packed column with specific solvent washes (toluene/hexane and dichloromethane).
- High-performance TLC was utilized for separation, with sulfonamides detected using fluorescamine spray reagent.
Main Results:
- The method achieved low detection limits, ranging from 0.07 to 0.44 ppm at the 99% confidence level for the five sulfonamides.
- Lowest detectable levels were as low as 0.04 ppm for sulfadiazine, sulfamethazine, sulfadimethoxine, and sulfapyridine.
- Average recoveries ranged from 57% to 63%, demonstrating good efficiency, though noted as analyst-dependent; linear responses were observed from 0 to 2 ppm.
Conclusions:
- The developed TLC method, incorporating MSPD, is effective for the sensitive and quantitative analysis of sulfonamide residues in salmon muscle.
- The method's low detection limits and linear response range make it suitable for routine monitoring and ensuring food safety.
- Further optimization may be needed to address analyst dependency in recovery rates for consistent results.