Related Experiment Video

Updated: Jul 11, 2026

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue
09:42

High-Resolution 3D Imaging of Rabies Virus Infection in Solvent-Cleared Brain Tissue

Published on: April 30, 2019

Autolyzed Brain Tissue as a Means of Facilitating Transmission of Experimental Poliomyelitis

A Milzer, C L Byrd

    Science (New York, N.Y.)
    |January 17, 1947
    PubMed

    Insights

    Autolyzed brain tissue diluent accelerates poliomyelitis virus transfer to animal models. This method successfully adapted the virus to mice, enabling isolation from human samples.

    Area of Science:

    • Virology
    • Animal Models
    • Infectious Diseases

    Background:

    • Poliomyelitis virus presents challenges in laboratory adaptation.
    • Efficient methods for virus transfer and animal model susceptibility are crucial for research.

    Purpose of the Study:

    • To evaluate the efficacy of autolyzed brain tissue diluent in poliomyelitis virus transmission.
    • To adapt the Leon strain of poliomyelitis virus to CFW Swiss mice.
    • To demonstrate the utility of this technique for isolating virus from human clinical samples.

    Main Methods:

    • Utilized autolyzed brain tissue diluent for virus preparation.
    • Inoculated poliomyelitis virus into CFW Swiss mice, hamsters, and rhesus monkeys.
    • Adapted the Leon monkey-passage strain to CFW Swiss mice.
    • Isolated poliomyelitis virus strains from human feces and spinal cord.

    Main Results:

    • Autolyzed brain tissue diluent significantly shortened incubation periods.
    • Successful adaptation of the Leon strain to CFW Swiss mice was achieved.
    • The technique enabled the isolation of multiple poliomyelitis virus strains from human sources in mice.

    Conclusions:

    • Autolyzed brain tissue diluent is an effective tool for enhancing poliomyelitis virus transfer to animal models.
    • CFW Swiss mice can be successfully infected and used for isolating poliomyelitis virus strains.
    • This method facilitates the study of poliomyelitis virus through improved animal model utility.
    Keywords:
    POLIOMYELITIS/experimental

    More Related Videos

    Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis09:01

    Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

    Published on: November 17, 2020

    Isolation of Brain-infiltrating Leukocytes06:44

    Isolation of Brain-infiltrating Leukocytes

    Published on: June 13, 2011

    Related Concept Videos

    Poliomyelitis01:17

    Poliomyelitis

    Poliomyelitis is caused by poliovirus, a small, non-enveloped, positive-sense RNA virus of the Picornaviridae family and Enterovirus genus. Transmission occurs primarily via the fecal-oral route, often through ingestion of contaminated water or food. The virus initially replicates in the oropharynx and intestinal mucosa, particularly in lymphoid tissues such as the tonsils, Peyer’s patches, and regional lymph nodes. Primary viremia follows, allowing dissemination throughout the body.In most...