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Updated: Jul 11, 2026

High-Resolution Comparison of Bacterial Conjugation Frequencies
Published on: January 10, 2019
Conjugative transfer of the IncP-7 carbazole degradative plasmid, pCAR1, in river water samples
Masaki Shintani1, Naoya Fukushima, Meguru Tezuka
1Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.
Abstract:
The transfer of the IncP-7 carbazole degradative plasmid pCAR1 from Pseudomonas putida SM1443 (derived from strain KT2440) into bacteria of river water samples was monitored using a reporter gene encoding red fluorescent protein (RFP). The number of transconjugants drastically increased in the presence of carbazole, and most appeared to belong to the genus Pseudomonas. The results suggest that the presence of carbazole benefits the appearance of transconjugants belonging to the genus Pseudomonas. Intriguingly, we also detected the transfer of pCAR1 into non-Pseudomonas, Stenotrophomonas-like bacteria.
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