Constituents and biological activity of Bidens pilosa L. grown in Egypt

Column chromotography and preparative TLC of the light petroleum-diethyl ether extract from Bidens pilosa L. afforded tridecapentyn-1-ene, trideca-2,12-diene-4,6,8,10-tetrayne-1-ol, trideca-3,11-diene-5,7,9-triyne-1,2-diol and trideca-5-ene-7,9,11-triyne-3-ol. The compounds were identified on the bases of UV, 1H-NMR spectra and comparison with reported data. The chloroform extract was chromatographed to yield B-amyrin, phytosterin-B, esculetin and B-sitosterol glucoside. The petroleum ether extract afforded long chain ester, saturated hydrocarbon, long chain alcohol, B-amyrin, phytosterin-B, lupeol and lupeol acetate. GLC analysis of the fatty acids indicated the presence of 5 acids. The antimicrobial test was carried out to indicate an evident activity. The antidiabetic activity showed non significant decrease of blood glucose.