Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Control of chondrocyte regulatory volume decrease (RVD) by [Ca2+]i and cell shape.

M J P Kerrigan1, A C Hall

  • 1School of Biosciences, Department of Human and Health Sciences, University of Westminster, London, UK. M.J.Kerrigan@wmin.ac.uk

Osteoarthritis and Cartilage
|September 15, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A potential key role for alpha-haemolysin of <i>Staphylococcus aureus</i> in mediating chondrocyte death in septic arthritis.

Bone & joint research·2018
Same author

A novel organ culture model of a joint for the evaluation of static and dynamic load on articular cartilage.

Bone & joint research·2018
Same author

Iatrogenic articular cartilage injury: the elephant in the operating theatre: the surgeons' role in chondroprotection.

The bone & joint journal·2017
Same author

Drying of open animal joints in vivo subsequently causes cartilage degeneration.

Bone & joint research·2016
Same author

Hyperosmolarity normalises serum-induced changes to chondrocyte properties in a model of cartilage injury.

European cells & materials·2016
Same author

Impact of bread making on fructan chain integrity and effect of fructan enriched breads on breath hydrogen, satiety, energy intake, PYY and ghrelin.

Food & function·2015
Same journal

Retsat promotes synovial mesenchymal stem cell senescence and aggravates osteoarthritis by inhibiting the PPARγ/RXR heterodimerization pathway.

Osteoarthritis and cartilage·2026
Same journal

Effectiveness of a blended care intervention in physiotherapy with exercise and education for patients with hip or knee osteoarthritis (SmArt-E): A multicentre pragmatic randomized controlled trial.

Osteoarthritis and cartilage·2026
Same journal

Through the lens of light sheet, knee innervation shines.

Osteoarthritis and cartilage·2026
Same journal

Deep learning-based quantification of knee effusion-synovitis volume on MRI - Technique development and validation.

Osteoarthritis and cartilage·2026
Same journal

Mapping articular cartilage maturation across postnatal development by proteomics.

Osteoarthritis and cartilage·2026
Same journal

Effect of an oral complementary medicine combination for symptomatic knee osteoarthritis: A double-blind, randomized, placebo-controlled trial.

Osteoarthritis and cartilage·2026
See all related articles

Articular chondrocytes regulate volume via calcium signaling, with 2D culture altering this response. This study clarifies the role of calcium and cell shape in chondrocyte volume regulation.

Area of Science:

  • Cell Biology
  • Biophysics
  • Biochemistry

Background:

  • Articular chondrocytes maintain matrix metabolism through controlled cell volume, regulated by membrane transporters.
  • The signaling pathways governing regulatory volume decrease (RVD) in response to cell swelling are not fully understood.
  • Calcium ions (Ca2+) and cell culture methods may influence chondrocyte volume regulation.

Purpose of the Study:

  • To investigate the role of Ca2+ in the regulatory volume decrease (RVD) of articular chondrocytes.
  • To determine the effects of 2D cell culture on chondrocyte volume regulation and associated Ca2+ signaling.
  • To elucidate the signal transduction pathways involved in chondrocyte volume homeostasis.

Main Methods:

  • Bovine articular chondrocytes, freshly isolated and 2D cultured, were subjected to hypotonic challenge.

Related Experiment Videos

  • Single-cell fluorescence microscopy was used to measure cell volume and intracellular calcium levels ([Ca2+]i).
  • The effects of calcium chelators (EGTA), specific channel blockers (REV5901), and Gd3+ were assessed; Ca2+ influx was evaluated using Mn2+ quench.

    • Main Results:

    • Freshly isolated chondrocytes showed robust RVD in ~50% of cells, inhibited by REV5901 and EGTA, but not Gd3+.
    • Hypotonic challenge induced a Gd3+-insensitive rise in [Ca2+]i, not consistently correlated with RVD.
    • 2D cultured chondrocytes exhibited Gd3+-insensitive RVD, but the associated [Ca2+]i rise was blocked by Gd3+.

    Conclusions:

    • The Ca2+ rise during hypotonic challenge is linked to RVD in some chondrocytes, but this relationship is complex.
    • 2D culture shifts the Ca2+ response to be Gd3+-sensitive, indicating altered signaling pathways.
    • Changes in chondrocyte shape due to 2D culture affect Ca2+ signaling, suggesting stretch-activated channels are not primary mediators of volume regulation in this context.