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Related Experiment Video

Updated: Jul 11, 2026

LED Thermo Flow — Combining Optogenetics with Flow Cytometry
05:49

LED Thermo Flow — Combining Optogenetics with Flow Cytometry

Published on: December 30, 2016

Practical time-gated luminescence flow cytometry. II: experimental evaluation using UV LED excitation.

Dayong Jin1, Russell Connally, James Piper

  • 1Centre for Lasers and Applications, Division of Information and Communication Sciences, Macquarie University, NSW, Australia. jin@ics.mq.edu.au

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|September 18, 2007
PubMed
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A new time-gated luminescence (TGL) flow cytometer using UV LED excitation effectively counts rare Europium-labeled targets. This method achieves high signal-to-background ratios, even in complex samples, demonstrating over 93% counting efficiency.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Optics

Background:

  • Time-gated luminescence (TGL) flow cytometry offers potential for rare-event detection.
  • Previous work explored UV LED excitation for TGL.
  • Autofluorescence and broadband signals pose challenges for conventional methods.

Purpose of the Study:

  • To construct and evaluate a practical TGL flow cytometer prototype.
  • To utilize UV LED excitation and a gated channel photomultiplier tube (CPMT) for enhanced detection.
  • To assess the system's performance in complex sample matrices.

Main Methods:

  • A compact TGL flow cytometer was built and optimized for a 6 kHz cycle rate.
  • Pulsed UV LED excitation (100 µs) followed by delay-gated detection (60 µs) was employed.

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  • Europium (Eu3+) luminescence beads were enumerated in autofluorescent water and S101 solutions.

    • Main Results:

    • The TGL flow cytometer successfully distinguished target beads from background noise.
    • A maximum signal-to-background ratio of 38:1 was achieved.
    • Counting efficiency exceeded 93% compared to conventional methods, with no background contribution from tested matrices.

    Conclusions:

    • The developed TGL flow cytometer is effective for rare-event detection in challenging samples.
    • UV LED excitation combined with gated detection provides high specificity and sensitivity.
    • This technology shows promise for applications requiring sensitive and selective cell counting.