Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.7K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spatial transcriptome for cell segmentation.

Nature computational science·2026
Same author

Hardware-Attentive Programmable Fourier Ptychography Enables Task-Adaptive Label-Free Virtual Staining.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Correction to "Thiolate DNAzymes on Gold Nanoparticles for Isothermal Amplification and Detection of Mesothelioma-derived Exosomal PD-L1 mRNA".

Analytical chemistry·2026
Same author

Binocular vision fusion enhanced 3D NIR-II <i>in vivo</i> imaging of bone and vessel networks.

Fundamental research·2026
Same author

Lattice and ligand engineering for hierarchical heterogeneous nanocrystals.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

White matter microstructure and cognitive decline in subacute ischemic stroke: Insights from free water imaging and glymphatic dysfunction.

Brain research bulletin·2026

Related Experiment Video

Updated: Apr 28, 2026

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-&#945;
08:26

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

11.9K

Beads-on-a-Tip testing for ultrasensitive antigen detection across a large dynamic range.

Ziwei Wu1, Yangjian Cai1, Yitong Zhao1,2

  • 1Institute for Biomedical Materials and Devices (IBMD), Faculty of Science University of Technology Sydney Sydney New South Wales Australia.

Smart Molecules : Open Access
|April 27, 2026
PubMed
Summary

This study introduces a novel Beads-on-a-Tip design for ultra-sensitive antigen detection, significantly improving lateral flow immunoassay (LFIA) sensitivity for point-of-care testing (POCT). The new method achieves a lower limit of detection than existing LFIAs.

Keywords:
Beads‐on‐a‐TipCOVID‐19rapid testingultrasenstive assayupconversion nanoparticles

More Related Videos

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM
11:02

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Published on: January 23, 2015

17.2K

Related Experiment Videos

Last Updated: Apr 28, 2026

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-&#945;
08:26

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

11.9K
Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays
07:13

Author Spotlight: High-Quality Quantum Dot Nanobeads for Sensitive Fluorescent Lateral Flow Immunoassays

Published on: June 28, 2024

2.2K
Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM
11:02

Detection of Exosomal Biomarker by Electric Field-induced Release and Measurement EFIRM

Published on: January 23, 2015

17.2K

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Point-of-Care Diagnostics

Background:

  • Lateral flow immunoassays (LFIAs) are widely used for point-of-care testing (POCT) due to their low cost and ease of use.
  • Current LFIAs often lack quantitative capabilities and suffer from limited sensitivity (nanogram range) due to passive fluidics and non-specific binding.
  • Nitrocellulose membranes in traditional LFIAs contribute to high background noise and reduced assay performance.

Purpose of the Study:

  • To develop an enhanced LFIA platform with improved sensitivity and quantitative capabilities for antigen detection.
  • To overcome the limitations of passive capillary fluidics and non-specific binding in conventional LFIAs.
  • To establish a novel diagnostic system for ultra-sensitive detection of biomarkers, using SARS-CoV-2 spike antigen as a model.

Main Methods:

  • A novel 'Beads-on-a-Tip' design was developed, replacing nitrocellulose membranes with magnetic beads in a pipette tip.
  • Capture antibodies were conjugated to magnetic beads for sandwich immunoassay, enabling active washing and enrichment of low-abundant antigens.
  • Upconversion nanoparticles (UCNPs) were utilized as luminescent reporters to enhance detection sensitivity.

Main Results:

  • The Beads-on-a-Tip design achieved a significantly reduced limit of detection (LOD) of 706 fg/mL for SARS-CoV-2 spike antigen.
  • The assay demonstrated a broad dynamic range spanning over 7 orders of magnitude (500 fg/mL to 10 μg/mL).
  • Optimization of incubation time and UCNP-to-bead ratio was crucial for enhancing immunocomplex formation and signal-to-noise ratio.

Conclusions:

  • The developed Beads-on-a-Tip LFIA system offers a substantial improvement in sensitivity and quantitative accuracy for POCT.
  • This novel approach effectively mitigates non-specific binding and background noise, leading to superior assay performance.
  • The system presents a promising solution for ultra-sensitive, quantitative diagnostics, particularly for low-abundant biomarkers.