Related Experiment Videos
Expression of enhancing factor gene and its localization in mouse tissues
R Mulherkar1, S J Desai, R S Rao
1Cell and Developmental Pathology Division, Tata Memorial Centre, Parel, Bombay, India.
Histochemistry
|January 1, 1991
Summary
Enhancing factor (EF), a protein involved in cell proliferation, is primarily synthesized in the Paneth cells of mouse intestines. This study confirms EF
Area of Science:
- Gastroenterology
- Cell Biology
- Molecular Biology
Background:
- Enhancing factor (EF) is a 14 kDa protein previously isolated from mouse small intestines.
- Prior research suggests EF plays a role in cell proliferation.
- Preliminary immunohistochemical data indicated EF localization within Paneth cells.
Purpose of the Study:
- To investigate the tissue distribution of Enhancing Factor (EF).
- To confirm the primary site of EF synthesis in the mouse small intestine.
- To support distribution data with molecular analysis.
Main Methods:
- Optimized immunohistochemical staining techniques for tissue distribution analysis.
- Northern blot analysis utilizing a specific nick-translated cDNA probe for EF.
- Analysis of gene transcription and protein localization.
Main Results:
- Immunohistochemistry revealed EF localization predominantly in Paneth cells at the base of crypts of Lieberkühn.
- Northern blot analysis confirmed active transcription of the EF gene primarily in intestinal tissues.
- Paneth cells identified as the main source of EF synthesis.
Conclusions:
- The Enhancing Factor (EF) gene is actively transcribed in the intestines.
- Paneth cells are the primary site for EF synthesis.
- EF's role in intestinal cell proliferation is further supported by its localization and synthesis site.