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Updated: Jul 11, 2026

Dissection and 2-Photon Imaging of Peripheral Lymph Nodes in Mice
Published on: August 23, 2007
Intravital two-photon imaging of T-cell priming and tolerance in the lymph node
Susanna Celli1, Philippe Bousso
1Départment d'Immunologie, Institut Pasteur, Paris, France.
Abstract:
Two-photon microscopy makes it possible to image in real-time fluorescently labeled cells located in deep tissue environments. We describe a procedure to visualize the behavior of lymph node T cells during either priming or tolerance, in live, anesthetized mice. Intravital imaging of T lymphocytes is a powerful tool to study the cellular orchestration of adaptive immune responses in physiological settings. This method should provide new insights into the regulation of lymphocyte migration and cell-cell interactions in various immunological contexts.
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