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Fluorescence-Based Detection of FEN1 Nuclease Activity and Screening of Small-Molecule Inhibitors
Published on: June 27, 2025
Metal-based netropsin mimics showing AT-selective DNA binding and DNA cleavage activity at red light
Ashis K Patra1, Tuhin Bhowmick, Suryanarayanarao Ramakumar
1Department of Inorganic and Physical Chemistry, and Bioinformatics Center, Department of Physics, Indian Institute of Science, Bangalore, India.
Abstract:
Copper(II) bis-arginate [Cu(l-arg)2](NO3)2 (1) and [Cu(l-arg)(phen)Cl]Cl (2) as mimics of the minor-groove-binding natural antibiotic netropsin show preferential binding to the AT-rich region of double-stranded DNA. The complexes with a d-d band near 600 nm display oxidative DNA cleavage activity on photoirradiation at UV-A light of 365 nm and at red light of 647.1 nm (Ar-Kr laser) in a metal-assisted photoexcitation process forming singlet oxygen (1O2) species in a type-2 pathway.
