Related Experiment Video

Updated: Jul 11, 2026

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

Published on: June 30, 2016

Involvement of macrophage colony-stimulating factor (M-CSF) in the function of follicular granulosa cells

Zhifen Zhang¹, Qin Fang, Jianmin Wang

¹Department of Gynecology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou, China. zhangzf_hz@hotmail.com

Fertility and Sterility

|September 21, 2007

Abstract

Objective:

To evaluate the role of macrophage colony-stimulating factor (M-CSF) in steroidogenesis by luteinized granulosa cells (GCs) and to examine the effect of FSH, E(2), and P on the production of M-CSF by GCs.

Design:

In vitro experimental study.

Setting:

University hospital with an IVF-intracytoplasmic sperm injection program.

Patient(S):

Forty infertile women who underwent intracytoplasmatic sperm injection.

Intervention(S):

The GCs obtained from these patients during oocyte retrieval were cultured and then treated with different concentrations of M-CSF, FSH, E(2), or P for 72 hours.

Main Outcome Measure(S):

The M-CSF concentrations were determined by ELISA, and the concentrations of E(2) and P were measured by enzyme immunoassay.

Result(S):

The M-CSF secretion of GCs was enhanced by FSH or E(2) in vitro in a dose-dependent manner but was unaffected by P. On the other hand, M-CSF elicited a production of E(2) and P by GCs in a dose-dependent manner in the presence or absence of FSH.

Conclusion(S):

The results suggest that the growth factor M-CSF might have an important role in ovarian function.

More Related Videos

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Effect of Anti-c-fms Antibody on Osteoclast Formation and Proliferation of Osteoclast Precursor In Vitro

Published on: March 18, 2019

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells

Isolation of Exosome-Enriched Extracellular Vesicles Carrying Granulocyte-Macrophage Colony-Stimulating Factor from Embryonic Stem Cells

Published on: November 11, 2021

Related Concept Videos

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Differentiation of Common Myeloid Progenitor Cells

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...

Folliculogenesis

Folliculogenesis

Folliculogenesis is the development of ovarian follicles, the specialized structures within the ovarian cortex where oogenesis, or egg development, occurs. This process is essential for female reproductive health and begins during fetal development when primordial follicles are formed. Each primordial follicle comprises a primary oocyte in the center, surrounded by a single layer of squamous pre-granulosa cells. These follicles remain dormant in late prophase I of meiosis until triggered by...

Role of Hematopoietic Growth Factors

Role of Hematopoietic Growth Factors

Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...

Articles linked to this work by shared authors, journal, and citation graph.

Wear and fracture of curettes due to sharpening and scaling processes.

International journal of dental hygiene·2021

Abundance of Lipopolysaccharide Heptosyltransferase I in Human Gut Microbiome and Its Association With Cardiovascular Disease and Liver Cirrhosis.

Frontiers in microbiology·2021

Clinicopathologic and molecular analysis of a BCOR-CCNB3 undifferentiated sarcoma of the kidney reveals significant epigenetic alterations.

Cold Spring Harbor molecular case studies·2021

Evaluation of the effects of adding activated carbon at different stages of composting on metal speciation and bacterial community evolution.

The Science of the total environment·2021

VideoDG: Generalizing Temporal Relations in Videos to Novel Domains.

IEEE transactions on pattern analysis and machine intelligence·2021

Ameliorating the hallmarks of cellular senescence in skeletal muscle myogenic progenitors in vitro and in vivo.

Science advances·2021

See all related articles