[Sensitivity of surface microorganisms to disinfectants]

The influence of humidity and temperature on survival of S. aureus and P. aeruginosa on the surfaces of titles, glass and blanket carriers has been estimated. The number of CFU was examined after exposure time 6 and 24 hours in temperatures of 21 degrees C, 37 degrees C and RH 35%, 95%. It was observed: 1. The important reduction of numbers of both microorganisms at temperature 37 degrees C and RH 95%, 2. The relatively high number of survival cells of P. aeruginosa on the surface of blankets at temp. 21 degrees C and RH 95%. The microorganisms on the carriers were previously kept for 24 h at temp. 21 degrees C, RH 35% and 95% and then exposed to solutions of chloramine, formalin, lysol and Sterinol (QAC). It was observed that there was a great dependence of the disinfecting effect on the degree of dessication of the surfaces. In all cases the resistance of contaminated carriers stored 24 h was higher at 95% RH than at 35% RH.