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[Isolation of a histone-specific proteinase from brain cell nuclei of rats]

A F Protas, P P Chaialo

Ukrainskii Biokhimicheskii Zhurnal (1978)

|September 1, 1991

Abstract:

A method for isolating histone proteinase of rat brain chromatin is described, including ammonium sulphate fractionation gel filtration on sefacryl 5200, ion exchange chromatography on mono S and affinity chromatography with benzamidine sefarose. The enzyme molecular weight equals 25 kDa. It is purified 15621 times in comparison with initial nuclear extract.

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